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[目的]研究煤焦沥青烟提取物(CTPE)刺激人支气管上皮细胞(BEAS-2B)的脂质过氧化作用。[方法]用不同浓度(0、1、3μg/mL)的CTPE刺激BEAS-2B细胞4 h后,流式细胞仪检测细胞活性氧(ROS)水平。刺激BEAS-2B细胞8 h后,用丙二醛(MDA)试剂盒和超氧化物歧化酶(SOD)活力测定试剂盒分别检测细胞裂解液中的MDA含量和SOD活力。[结果]与对照组相比,CTPE(1、3μg/mL)作用细胞4 h后,ROS含量均显著升高(P<0.05)。1、3μg/mL CTPE刺激细胞8 h后,MDA含量均升高,SOD活力均明显降低(P<0.05);MDA/SOD值升高(P<0.05)。[结论]煤焦沥青烟提取物刺激BEAS-2B细胞后ROS水平和MDA含量增高,SOD活力降低,发生了脂质过氧化作用。
[Objective] To investigate the lipid peroxidation of human bronchial epithelial cells (BEAS-2B) stimulated by coal tar pitch smoke extract (CTPE). [Method] BEAS-2B cells were stimulated with CTPE at different concentrations (0, 1, and 3μg / mL) for 4 h. The level of reactive oxygen species (ROS) was measured by flow cytometry. After BEAS-2B cells were stimulated for 8 h, MDA content and SOD activity in cell lysate were detected by using MDA kit and SOD activity assay kit respectively. [Result] Compared with the control group, the content of ROS in CTPE (1, 3μg / mL) treated cells increased significantly after 4 h (P <0.05). After stimulated by 1,3,5 μg / mL CTPE for 8 h, MDA content increased, SOD activity decreased significantly (P <0.05) and MDA / SOD increased (P <0.05). [Conclusion] The lipid peroxidation of BEAS-2B cells induced by coal tar pitch smoke extract increased ROS level and MDA content, decreased SOD activity.