建立了水溶性荧光素-十六烷基三甲基溴化铵测定阿莫西林含量的荧光探针法。在pH 8.60的Sфrensen缓冲溶液中,十六烷基三甲基溴化铵(CTMAB)与水溶性荧光素(SF)发生荧光减弱反应,加入阿莫西林后,体系荧光强度增强,且加入量与增强程度呈良好线性关系。在优化的条件下,线性范围为0.006～0.72 mg/L,检出限为0.0018 mg/L。检测阿莫西林在动物血清中的含量,回收率为98.4%～100.8%,相对标准偏差在1.6%～3.2%之间。 A fluorescent probe method for the determination of amoxicillin with water-soluble fluorescein-cetyltrimethylammonium bromide was established. In pH 8.60 Sфrensen buffer solution, cetyltrimethylammonium bromide (CTMAB) and water-soluble fluorescein (SF) fluorescence decreased reaction, adding amoxicillin, the system fluorescence intensity increased, and the amount and The degree of enhancement shows a good linear relationship. Under the optimized conditions, the linear range was 0.006 ~ 0.72 mg / L with the detection limit of 0.0018 mg / L. The detection of amoxicillin in animal serum content, recovery was 98.4% to 100.8%, the relative standard deviation of 1.6% to 3.2%.