目的　诱导EDA基因在原核细胞中的表达。方法　对 pCMV -EDA -A1、pGEX -4T -3进行酶切 ,回收目的片段连接 ,获得重组质粒 pGEX -4T -3 -EDA ;用IPTG诱导 pGEX -4T -3 -EDA在大肠杆菌DH5α中表达。结果　EDA基因可在大肠杆菌DH5α中表达。随着诱导时间的延长 ,EDA基因的表达量增加。重组EDA存在于包涵体中。结论　EDA基因可以在原核细胞中获得表达 ,为深入研究该蛋白在牙齿发育中的具体作用奠定了基础 Objective To induce the expression of EDA gene in prokaryotic cells. Methods The pCMV-EDA -A1 and pGEX-4T-3 were digested and ligated to obtain the recombinant plasmid pGEX-4T-3-EDA. IPTG was used to induce pGEX-4T-3-EDA expression in E. coli DH5α. Results The EDA gene can be expressed in E. coli DH5α. With the induction of time, EDA gene expression increased. Recombinant EDA is present in inclusion bodies. Conclusion EDA gene can be expressed in prokaryotic cells, which lays the foundation for further study on the specific role of this protein in tooth development