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目的 建立大鼠穿透性角膜移植排斥动物模型 ,总结模型制作中的并发症出现原因和相应对策 ,以及增加异体移植排斥率的注意事项。方法 采用远交系Wistar雌性大鼠和SD雌性大鼠建立穿透性角膜移植排斥模型。为了提高建立异体角膜移植排斥模型的成功率 ,在取供体角膜植片时靠近角膜缘取材 ,术后保留角膜缝线。结果 自体角膜移植组无排斥反应发生 ,异体角膜移植组角膜植片存活时间为 10 8d± 2 1d ,排斥率为 10 0 %。两组 6 8例模型术后并发症有虹膜前粘连 5只 ,虹膜脱出 5只 ,眼内出血 1只 ,白内障 3只和眼内感染 1只。结论 实验中采用的大鼠角膜移植模型 ,其临床表现与人类角膜移植排斥反应相似 ,排斥反应发生较早且排斥率高 ,适用于模拟人类角膜移植排斥现象进行深入的相关临床和基础研究。轻柔熟练的显微操作技术 ,锐利的手术器械 ,以及充分散大的瞳孔可以最大程度地减少术后并发症的发生。选择组织相容性抗原间差别大的品系、偏中心移植和术后保留缝线是增加移植排斥率的关键措施
OBJECTIVE: To establish an animal model of rat penetrating keratoplasty rejection, to summarize the causes of the complication in the model making and the corresponding countermeasures, as well as to increase the rejection rate of allograft. Methods Penetrating keratoplasty rejection model was established in outbred Wistar female rats and SD female rats. In order to improve the success rate of establishing a rejection model of allograft keratoplasty, the donor corneal graft was taken near the limbus and the corneal suture was preserved after operation. Results No rejection occurred in autologous corneal transplantation group. The survival time of corneal graft in allogeneic corneal transplantation group was 108 days ± 21 days, and the rejection rate was 100%. There were 5 cases of pre-iris adhesion, 5 cases of iris prolapse, 1 case of intraocular hemorrhage, 3 cases of cataract and 1 case of intraocular infection. Conclusion The rat corneal transplantation model used in this study is similar to human keratoplasty rejection, with early rejection and high rejection rate. It is suitable for the clinical and basic research of simulating human keratoplasty rejection. Gentle and skilled micromanipulation techniques, sharp surgical instruments, and ample pupil pupil can minimize the incidence of postoperative complications. The selection of histocompatibility between the different strains of different antigens, partial center graft and postoperative retention of suture is to increase the rate of graft rejection, the key measures