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以高抗大豆孢囊线虫４号生理小种的２个大豆品系１２５９系（黄色）和１２５９系（双色）及其抗感亲本灰皮支黑豆和晋遗９号，以及另外两个高抗品种ＰＩ４３７６５４和元钵黑豆，两个高感品种鲁豆１号和鲁豆７号为试验材料，进行大豆抗孢囊线虫的ＰＡＰＤ分析。在所用的３３个引物中，引物ＯＰＧ０４的扩增产物中有一条谱带系抗病品系、抗病品种（包括抗病亲本）特有。
Two soybean lines 1259 (yellow) and 1259 (two-color) lines with resistance to susceptible parents Pigeonuodongshu and Jinyu 9 and two other high resistant varieties PI437654 and Yuanpao black beans, two high susceptible varieties Luodou 1 and Luding 7 were used as materials for PAPD analysis of soybean against cyst nematode. Of the 33 primers used, one of the amplified products of the primer OPG04 has a lineage-resistant strain that is peculiar to resistant varieties (including resistant parents).