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目的:了解法国新喀里多尼亚地区蜱中贝氏考克次体的流行状况。方法:以贝氏考克次体的16SrRNA为靶基因,病原体的RNA自蜱中提取,使用荧光定量PCR技术,检测法国新喀里多尼亚地区三种蜱共456株中贝氏考克次体的流行率。结果:以30为CT值,共检测出阳性334株,阳性率73.2%。按蜱种分类:硬蜱虫2株皆为阴性;血红扇头蜱阳性163株,阳性率69.9%;边缘革蜱阳性171株,阳性率77.4%。结论:法国蜱中贝氏考克次体的流行率很高,不同种蜱阳性率不同。
OBJECTIVES: To understand the epidemiology of bivalve ciconia in ticks in New Caledonia, France. Methods: 16S rRNA of Bayesian cynomolgus was taken as target gene and RNA of pathogen was extracted from tick. Fluorescence quantitative PCR was used to detect 456 strains of three ticks in New Caledonia in France. The prevalence of the body. Results: A total of 334 positive strains were detected with a CT value of 30, with a positive rate of 73.2%. According to the species of ticks, 2 strains of hard ticks were negative, 163 were positive for black ticks, the positive rate was 69.9%, and 171 were positive for ticks, the positive rate was 77.4%. CONCLUSION: The prevalence of Cryptosporidium bifidum in French ticks is very high, and the positive rates of different species of ticks are different.