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在豌豆根腐病病原研究中,采用了PDA、WA组织分离、胡萝卜诱饵分离及水培养分离等不同的分离技术,共分离病株820株,病组织1734块,获得菌株235个,经初步鉴定确定为17个不同的种,对这些分离物采用灭菌土盆栽、蛭石盆栽、纸卷法、断根法等接种技术进行致病性测定。根据分离率和致病性测定结果,认为发生在甘肃中部地区的豌豆根腐病是由茄镰刀菌(Fusarium solani(Mars)Sacc)、豌豆丝囊霉(Aphanomyces euteiches)、链孢粘帚霉(Gliocladium catenulatum Gilman et Abbott)、尖孢镰刀菌(Fusarium oxysporum Schlecht)、根串珠霉(Thielaviopsis basicola)、立枯丝核菌(Rhizoctonia solani Kuhn)、腐霉(Pythium spp)和壳二孢菌(Ascochyta spp)等复合侵染引起的。根据文献记载,上述各类菌除茄镰刀菌、尖孢镰刀菌和丝囊霉之外,其余真菌在豌豆上的致病性国内未见报道,链孢粘帚霉作为豌豆根腐病的致病菌为国内外首次报道。
In pea root rot pathogen study, using PDA, WA tissue separation, carrot bait separation and water culture separation of different separation techniques, a total of 820 strains of diseased plants, diseased tissue 1734, obtained strains of 235, preliminary identification Seventeen different species were identified, and the pathogenicity of these isolates was determined by using inoculation techniques such as sterilized potted soil, vermiculite pot, paper roll method, and root pruning method. Based on the results of the isolation rate and pathogenicity test, pea root rot occurred in central Gansu province was determined by Fusarium solani (Mars) Sacc, Aphanomyces euteiches, Gliocladium catenulatum Gilman et Abbott, Fusarium oxysporum Schlecht, Thielaviopsis basicola, Rhizoctonia solani Kuhn, Pythium spp and Ascochyta spp ) And other complex infections caused. According to the literature, the above-mentioned types of bacteria except Fusarium solani, Fusarium oxysporum and Sarcandra other than the pathogenicity of the rest of the fungus on peas has not been reported, Gliocladium spirotricarum as pea root rot caused The bacteria are the first reported at home and abroad.