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通过对玉米与四倍体多年生玉米杂种F1采用遮光调节开花时期、喷施低浓度赤霉素等提高可杂交性措施 ,选育出一个含有四倍体多年生玉米遗传物质的、在形态特性与普通玉米相似的种质 ,并对供试材料的表型性状进行了调查 ,对玉米及其与四倍体多年生杂交后代材料的体细胞染色体进行多色基因组原位杂交 (Multi colorGenomeinsituHybridization ,McGISH)检测。结果表明 :玉米与四倍体多年生玉米种杂交后代F2 ×P1的许多表型性状已经回复到玉米的特征 ;F2 ×P1自交一代 (BC1F3 )染色体数目为 2 0 ,在原位杂交检测的植株中有 17条与玉米亲本显色相同 ,另外 3条染色体区别于玉米基因组 ,检出为红色荧光且形态上小于玉米染色体 ,表明其来源于四倍体多年生玉米染色体。可见 ,BC1F3 是玉米 四倍体多年生玉米异源代换种质。
Through the hybridization of corn and tetraploid perennial corn hybrid F1 with shading to adjust the flowering period and the spraying of low concentration of gibberellin to improve the hybridity, a tetraploid perennial maize genetic material was bred and its morphological characteristics and common The similar germplasms of maize were investigated. The phenotypic traits of the tested materials were investigated. The multicolor genome in situ hybridization (McGISH) was used to detect somatic chromosomes of maize and tetraploid perennial hybrid progeny. The results showed that many phenotypic traits of F2 × P1 cross of offspring of maize and tetraploid perennial maize had returned to maize characteristics. The number of chromosomes of F2 × P1 selfing (BC1F3) was 20, 17 of them had the same color as maize parents. The other 3 chromosomes were distinguished from maize genome and detected as red fluorescence and were smaller in morphology than maize chromosomes, indicating that they originated from tetraploid perennial maize chromosomes. Can be seen, BC1F3 is tetraploid perennial corn heterologous substitution germplasm.