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为研制蝮蛇抗栓酶的换代产品，开发单一组分的类凝血酶制剂。采用离子交换和亲和层析等现代纯化方法，从东北白眉蝮蛇蛇毒中分离出了单一组分的类凝血酶。半成品经高效液相色谱凝胶柱测定，纯度大于90％，最高可达97.94％，分子量38200D，SDS－PAGE电泳测定为一条带，分子量为42100D，活性为1137BU／mg，比活性为1705BU／mg·pro，不含出血毒和神经毒，冻干成品制剂全部符合注射用品标准。
For the development of viper antithrombin replacement products, the development of single-component thrombin-like preparations. A single component of thrombin-like protein was isolated from the snakehead snakehead snake venom using ion exchange and affinity chromatography and other modern purification methods. The semi-finished product was determined by high performance liquid chromatography gel column with a purity of more than 90%, a maximum of 97.94% and a molecular weight of 38200D. The molecular weight of the semi-finished product was 42100D with an activity of 1137BU / mg and a specific activity of 1705BU / mg · Pro, does not contain bleeding poison and nerve poison, freeze-dried finished products all comply with injection standards.