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用水稻（ Ｏｒｙｚａ ｓａｔｉｖａ Ｌ．） 的幼胚、愈伤组织作受体，采用ＰＩＧ 基因枪法，成功地将苏云金杆菌毒蛋白基因［Ｂｔ 毒蛋白基因，ｃｒｙＩＡ（ｂ）］ 和马铃薯蛋白酶抑制剂基因（ ｐｉｎ ＩＩ基因） 连同抗除草剂Ｂａｒ 基因分别转入不同的水稻中，获得大量的转基因植株。Ｓｏｕｔｈｅｒｎ 杂交分析表明两种外源抗虫基因均分别整合到水稻的基因组中。Ｎｏｒｔｈｅｒｎ 杂交分析显示它们在水稻叶片中表达的水平较高。外源基因在转基因后代中遵循３∶１ 的孟德尔分离规律。初步的抗虫测试结果证明两种转基因株系均具有较强的抗虫性。本研究为进一步获得双价抗虫和抗除草剂水稻品系奠定了基础。
The immature embryos and callus of rice (Oryza sativa L.) were used as recipients. Bacillus thuringiensis toxin gene [Bt toxin gene, cryIA (b)] and potato protease inhibitor gene pin II gene) together with the herbicide-resistant Bar gene were transferred into different rice, access to a large number of transgenic plants. Southern hybridization analysis showed that the two exogenous insect-resistant genes were integrated into the genome of rice. Northern blot analysis showed that they are highly expressed in rice leaves. Exogenous gene in the transgenic progeny to follow the 3: 1 Mendelian separation. The preliminary results of pest resistance test showed that both transgenic lines had strong insect resistance. This study laid the foundation for further obtaining bivalent insect-resistant and herbicide-resistant rice lines.