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目的:研究环境内分泌干扰物双酚A(BPA)对SD大鼠血-脑屏障成分表达及星形胶质细胞活力的影响并探讨其损伤机制。方法:通过MTT检测细胞活力,高效液相色谱法(HPLC)检测谷氨酸含量的变化,实时荧光定量PCR检测星形胶质细胞中Claudin-3、Claudin-4、Claudin-5、JAM-1、JAM-2、ZO-1、E-cadherin基因的表达。结果:10-7 mg/ml的BPA与星型胶质细胞孵育24或48 h后即可引起细胞活力的下降,具有统计学差异。在该浓度的暴露下,血-脑屏障紧密连接相关蛋白Claudin-3、Claudin-4、JAM-1、E-cadherin蛋白的mRNA水平下调,而Claudin-5、JAM-2、ZO-1蛋白基因表达上调且具有统计学意义;HPLC结果显示,谷氨酸的浓度上调。结论:环境内分泌干扰物BPA对血-脑屏障的完整性具有潜在的损伤作用。
Objective: To study the effect of environmental endocrine disruptor bisphenol A (BPA) on the expression of blood-brain barrier and the activity of astrocytes in SD rats and to explore its mechanism. Methods: The activity of glutamate was detected by MTT and the content of glutamate in the astrocytes were detected by real-time fluorescence quantitative PCR. The levels of Claudin-3, Claudin-4, Claudin-5 and JAM-1 , JAM-2, ZO-1, E-cadherin gene expression. Results: BPA at 10-7 mg / ml could induce a decrease in cell viability after 24 or 48 h incubation with astrocytes, with statistical significance. At this concentration, the mRNA levels of Claudin-3, Claudin-4, JAM-1 and E-cadherin in the blood-brain barrier were down-regulated, while the expressions of Claudin-5, JAM-2 and ZO- The expression was up-regulated and statistically significant; HPLC results showed that the concentration of glutamate was increased. Conclusion: The environmental endocrine disruptor BPA has the potential damage effect on the integrity of blood-brain barrier.