论文部分内容阅读
2014—2015年在新疆沙湾县加工型辣椒Capsicum annuumL.上发现一种细菌性叶斑病。为明确其病原种类,采用组织分离法分离病原菌,依据柯赫氏法则进行致病性测定,并对菌株进行16S rDNA和持家基因rpoD及gry B的扩增、序列测定和系统发育分析,对其进行鉴定。结果表明,获得的8个细菌菌株间致病力无明显差异。致病细菌与已报道的黄褐假单胞菌Pseudomonas fulva菌株的16S rDNA、rpoD基因、gry B基因的同源性分别达到了99.1%~99.9%、99.4%~99.5%和97.6%~100.0%。结合革兰氏染色反应、菌体形态、培养性状、生理生化反应、寄主范围等特征,将病原菌鉴定为黄褐假单胞菌。该病原菌通过人工接种还能侵染番茄、茄子、马铃薯、黄瓜、西瓜、甜瓜、四季豆、豆角、豇豆、白菜、萝卜、胡萝卜及芹菜等多种植物。
A bacterial leaf spot was found on processed Capsicum annuum L. from Shawan County, Xinjiang in 2015-2015. To clarify the pathogen species, the pathogen was isolated by tissue isolation, pathogenicity was determined by Koch’s rule, and the 16S rDNA and housekeeping genes rpoD and gry B were amplified, sequenced and phylogenetically analyzed. Identification. The results showed that there was no significant difference in pathogenicity between the eight bacterial strains obtained. The homology of the 16S rDNA, rpoD gene and gry B gene of pathogenic bacteria with reported Pseudomonas sp. Strains was 99.1% -99.9%, 99.4% -99.5% and 97.6% -100.0%, respectively. . The pathogen was identified as Pseudomonas putida by combining with Gram stain reaction, cell morphology, culture traits, physiological and biochemical reactions and host range. The pathogen through inoculation can also infect tomato, eggplant, potato, cucumber, watermelon, melon, green beans, beans, cowpea, cabbage, radish, carrot and celery and other plants.