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目的：克隆小鼠牙本质磷蛋白（ＤＰＰ）ｃＤＮＡ。方法：用异硫氰酸胍一步法从小鼠牙胚组织中抽提总ＲＮＡ，用ｏｌｉｇｏ（ｄｔ） 作引物逆转录合成牙胚ｃＤＮＡ，然后利用ＰＣＲ 方法，从ｃＤＮＡ 中扩增出小鼠ＤＰＰｃＤＮＡ基因片段（ 约１ ．６ｋｂ），将所得基因片段插入ｐＢｌｕｅｓｃｒｉｐｔ 质粒载体，转化到大肠杆菌ＸＬ１ － Ｂｌｕｅ 后挑选阳性克隆，提取重组质粒ＤＮＡ，通过酶切分析和核苷酸序列分析鉴定阳性克隆。结果：酶切图谱和部分序列分析结果与国外文献报道一致。结论：克隆到小鼠ＤＰＰｃＤＮＡ 基因片段。
Objective: To clone mouse dentin phosphoprotein (DPP) cDNA. METHODS: Total RNA was extracted from mouse tooth germ tissue by one-step guanidine isothiocyanate method, reverse transcription of oligodeoxynucleotide (DTT) was used to synthesize tooth germ cDNA, and then PCR was used to amplify mouse DPP cDNA The fragment was inserted into pBluescript plasmid vector and transformed into E. coli XL1 - Blue. The positive clones were selected and the recombinant plasmid DNA was extracted. The positive clones were identified by restriction analysis and nucleotide sequence analysis. Results: The results of enzyme digestion and partial sequence analysis were consistent with those reported in foreign literature. Conclusion: The mouse DPP cDNA gene fragment was cloned.