论文部分内容阅读
目的：观察粉防己碱（Ｔｅｔ）对培养大鼠心肌细胞内游离钙（［Ｃａ￣（２＋）］ｉ）的影响。方法：运用Ｃａ￣（２＋）指示剂Ｆｕｒａ－２作为细胞内钙离子的荧光探针，利用ＡＲ－ＣＭ－ＭＩＣ阳离子测定系统，检测培养乳鼠心肌细胞内游离钙的浓度（［Ｃａ￣（２＋）］ｉ）。结果：在细胞外液钙浓度为１．８ｍｍｏｌ／Ｌ时，心肌细胞静息［Ｃａ￣（２＋）］ｉ为８０．３±６．９ｍｍｏｌ／Ｌ。在细胞外液钙浓度为１．８～５．４ｍｍｏｌ／Ｌ时，粉防己碱（Ｔｅｔ）对细胞静息［Ｃａ￣（２＋）］ｉ无明显影响。Ｔｅｔ１～１００μｍｏｌ／Ｌ剂量依赖的抑制高钾和Ｈ＿２Ｏ＿２引起的［Ｃａ￣（２＋）］ｉ的升高，Ｔｅｔ１０μｍｏｌ／Ｌ能对抗去甲肾上腺素引起的［Ｃａ￣（２＋）］ｉ升高。对搏动细胞，Ｔｅｔ能抑制细胞外高钙引起的收缩期［Ｃａ￣（２＋）］ｉ瞬间的变化，减慢搏动频率。结论：Ｔｅｔ对高Ｋ￣＋、去甲肾上腺素、Ｈ＿２Ｏ＿２及高钙引起的［Ｃａ￣（２＋）］ｉ增高的抑制作用可能是其抗心肌缺血机理之一。
Objective: To observe the effect of tetrandrine on intracellular free calcium ([Ca ~ (2 +)] i in cultured rat myocardial cells. Methods: Fura-2, a Ca 2+ indicator, was used as fluorescent probe of intracellular calcium ion. The concentration of intracellular free calcium ([Ca 2+ (2+ )] I). Results: When the concentration of extracellular calcium was 1.8mmol / L, the resting [Ca ~ (2 +)] i of cardiomyocytes was 80.3 ± 6.9mmol / L. Tetracycline (Tet) had no effect on cell resting [Ca ~ (2 +)] i when extracellular calcium concentration was 1.8-5.4 mmol / L. Tet1-100μmol / L dose-dependently inhibited the increase of [Ca ~ (2 +)] i induced by high potassium and H_2O_2, while Tet10μmol / L could antagonize the increase of [Ca ~ (2 +)] i induced by norepinephrine. For beating cells, Tet can inhibit the extracellular calcium-induced systolic [Ca ~ (2 +)] i transient changes, slowing down the pulsatile frequency. Conclusion: The inhibitory effect of Tet on the increase of [Ca ~ (2 +)] i induced by high K ~ +, norepinephrine, H_2O_2 and high calcium may be one of the mechanisms of its anti - myocardial ischemia.