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目的:RA538是从维甲酸诱导终末分化的人食管癌细胞系中分离出的一个与分化相关的基因,本研究探讨了其抗癌作用.方法:利用腺病毒介导基因转移观察对肿瘤细胞的抑制效应.结果:通过重组体腺病毒将RA538基因转导到人肺腺癌细胞系(GLC-82)和人结肠癌细胞系(HCY)中,获得较高的转导效率,显示出较明显的抑制肿瘤生长作用,抑制率分别为77.2%和77.9%,并能降低两细胞系体内肿瘤形成能力.流式细胞计数、DNA片段化分析及TUNEL检测证实RA538可诱导肿瘤细胞发生凋亡.Westem blot分析表明,RA538具有明显下调c-myc基因表达的作用,结论:研究结果提示,RA538是一个较广谱的抑癌基因,可以作为肿瘤基因治疗的一个新目的基因,具有良好的应用前景.
OBJECTIVE: RA538 is a differentiation-related gene isolated from human esophageal cancer cell lines induced by retinoic acid in terminally differentiated cells. This study explored its anti-cancer effects. Methods: Using adenovirus-mediated gene transfer to observe tumor cells Inhibitory effect. Results: Transduction of RA538 gene into human lung adenocarcinoma cell line (GLC-82) and human colon cancer cell line (HCY) by recombinant adenovirus resulted in higher transduction efficiency, showing a The inhibition of tumor growth was markedly inhibited by 77.2% and 77.9%, respectively, and the ability of tumor cell formation in both cell lines was reduced. Flow cytometry, DNA fragmentation analysis and TUNEL assay confirmed that RA538 can induce apoptosis of tumor cells. Westem blot analysis showed that RA538 can significantly down-regulate the expression of c-myc gene. Conclusion: The results suggest that RA538 is a broad-spectrum tumor suppressor gene and can be used as a novel target gene for tumor gene therapy, with good application prospects. .