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目的建立同时测定知母中新芒果苷,芒果苷,知母皂苷BⅡ,2,6,4’-三羟基-4-甲氧基苯甲酮,构树宁B,顺-扁柏树脂酚的UPLC方法。方法采用ACQUITY UPLC,HSS T3色谱柱(2.1 mm×100 mm,1.8μm),流动相为乙腈-0.03%的磷酸水溶液梯度洗脱,流速为0.5 mL.min-1,检测波长采用210和192 nm。结果新芒果苷,芒果苷,知母皂苷BⅡ,2,6,4’-三羟基-4-甲氧基苯甲酮,构树宁B,顺-扁柏树脂酚的质量浓度与峰面积分别在15.00～299.6μg.mL-1,8.80～175.6μg.mL-1,40.00～800.0μg.mL-1,2.300～45.60μg.mL-1,0.420～8.50μg.mL-1,0.400～9.00μg.mL-1内呈良好的线性关系。平均加样回收率(n=9)均在97.7%～100.6%之间,RSD均小于1.2%。结论该方法快速、准确、简便,可作为同时测定知母中新芒果苷,芒果苷,知母皂苷BⅡ,2,6,4’-三羟基-4-甲氧基苯甲酮,构树宁B,顺-扁柏树脂酚的方法。
OBJECTIVE To establish a UPLC method for simultaneous determination of neogenin, mangiferin, timosaponin BⅡ, 2,6,4’-trihydroxy-4-methoxybenzophenone, method. Methods The ACQUITY UPLC and HSS T3 columns (2.1 mm × 100 mm, 1.8 μm) were used. The mobile phase consisted of a gradient of acetonitrile-0.03% phosphoric acid. The flow rate was 0.5 mL.min-1. The detection wavelength was set at 210 and 192 nm . Results The mass concentration and peak area of nerol mangiferin, mangiferin, timosaponin BⅡ, 2,6,4’-trihydroxy-4-methoxybenzophenone, Zuoshuning B and cis-hinokitiol were 15.00-299.6 μg.mL-1, 8.80-175.6 μg.mL-1, 40.00-800.0 μg.mL-1, 3.00-45.60 μg.mL-1, 0.420-8.50 μg.mL-1, and 0.400-9.00 μg. mL-1 showed a good linear relationship. The average recoveries (n = 9) ranged from 97.7% to 100.6% with RSDs less than 1.2%. Conclusion The method is rapid, accurate and simple and can be used for the simultaneous determination of ne mangiferin, mangiferin, timosaponin B Ⅱ, 2,6,4’-trihydroxy-4-methoxy benzophenone, B, cis - Cypress resin phenol method.