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目的 探讨压力超负荷下心肌细胞凋亡对兔左室重构中的作用。方法 采取末端脱氧核苷酸转换酶介导的dUTP 生物素平移末端标记技术 ,观察持续压力超负荷引起兔心力衰竭的左室心肌细胞凋亡的动态变化。结果 对照组罕见凋亡细胞 ,腹主动脉狭窄术后第 1天凋亡细胞明显高于对照组 (P <0 0 0 1) ,主要分布在心内膜下和乳头肌 ;术后第 3天与术后第 7天之间存在一个峰值 ,凋亡细胞主要分布在心内膜下、乳头肌和心肌中层 ;术后第 14天凋亡数下降 ,但仍与对照组相比存在统计学意义 (P <0 0 5 )。心衰出现后凋亡细胞数再次明显增高 ,与其它各组比较均存在显著性差异 (P <0 0 0 1)。结论 慢性压力超负荷持续存在时 ,凋亡细胞可能参与了左室重构的调节
Objective To investigate the effect of cardiomyocyte apoptosis on left ventricular remodeling in rabbits under pressure overload. Methods The terminal deoxynucleotidyl transferase mediated dUTP biotin translocation end labeling was used to observe the dynamic change of left ventricular myocyte apoptosis induced by continuous pressure overload in rabbit heart failure. Results Apoptotic cells were rare in the control group. The number of apoptotic cells on the 1st day after abdominal aortic stenosis was significantly higher than that of the control group (P <0.01 01), mainly distributed in the subendocardium and papillary muscle. On the 3rd day after operation There was a peak between the 7th postoperative day and the apoptotic cells were mainly located in the subendocardium, papillary muscle and myocardium. The number of apoptotic cells decreased on the 14th postoperative day, but there was still statistical significance compared with the control group (P <0 0 5). The number of apoptotic cells increased significantly after heart failure, which was significantly different from other groups (P <0.01). Conclusion Apoptotic cells may participate in the regulation of left ventricular remodeling when chronic stress overload persists