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对源于我国黑龙江省佳木斯市同一块茄子田的14个核盘菌(Sclerotinia sclerotiorum)菌株及其中1个菌株Ep-1PNA5的2个衍生菌株的致病力和菌丝中的双链RNA(dsRNA)进行了分析。在马铃薯琼脂培养基(PDA)上对5个弱致病型核盘菌菌株(Ep-1PD、Ep-1PI、Ep-1PL、Ep-1PM、Ep-1PN)异常性状(菌丝生长慢,且异常分枝)的传染特性进行了测定。结果表明:在离体油菜叶片上,16个供试核盘菌菌株中,7个属于强致病类型,7个属于弱致病类型,2个属于中等致病类型。从10个核盘菌菌株的菌丝中检测到dsRNA因子,并可分成3类dsRNA电泳谱型。第一类dsRNA谱型只含有7.4kb大小的dsRNA分子,3个强致病型核盘菌菌株属于这种谱型;第二类dsRNA谱型含有2种dsRNA分子,大小分别为6.4kb和7.4kb。6个弱致病型菌株属于这种谱型;第三类dsRNA谱型只含有6.4kb大小的dsRNA分子。1个弱致病类型菌株属于这种谱型。从另外6个核盘菌菌株的菌丝中没有检测到任何dsRNA因子,其中4个菌株属于强致病型菌株,2个菌株属于中等致病型。可见,6.4kb大小的dsRNA因子与核盘菌弱致病性状密切相关。5个弱致病型核盘菌菌株(Ep-1PD、Ep-1PI、Ep-1PL、Ep-1PM、Ep-1PN)的异常性状可以通过菌丝接触传染给一些强致病型核盘菌菌株,使其菌丝生长变慢及分枝异常。结果还表明:这些弱致病型核盘菌异常性状的传染具有菌株特异性。
The pathogenicity of 14 strains of Sclerotinia sclerotiorum and one of the 2 strains derived from Ep-1PNA5 from the same eggplant field in Jiamusi, Heilongjiang Province, China, and the double-stranded RNA (dsRNA )Analyzed. The abnormal traits (slow mycelial growth, and mycorrhizal fungi growth) of the five attenuated S. sclerotiorum strains (Ep-1 PD, Ep-1 PI, Ep-1 PL, Ep-1 PM, Ep-1 PN) on potato agar medium Abnormal branch) of the infectious characteristics were measured. The results showed that among the 16 tested S. sclerotiorum isolates, 7 were strongly pathogenic, 7 were weak pathogenic and 2 were intermediate pathogenicity. DsRNAs were detected from the mycelia of 10 S. sclerotiorum strains and classified into three types of dsRNA electrophoretic patterns. The first dsRNA profile contained only 7.4 kb dsRNA molecules and three strongly pathogenic S. sclerotiorum strains belonged to this pattern. The second dsRNA profile contained two dsRNA molecules, 6.4 kb in size and 7.4 kb. Six weakly pathogenic strains belonged to this pattern; the third type of dsRNA profile contained only a 6.4kb dsRNA molecule. A weak pathogenic strain belongs to this genotype. No dsRNAs were detected in mycelia of 6 other S. sclerotiorum strains, of which 4 belonged to strongly pathogenic strains and 2 strains belonged to moderately pathogenic ones. Can be seen, 6.4kb size of dsRNA factor is closely related to the weak pathogenicity of Sclerotinia. Abnormal traits of the five attenuated S. sclerotiori strains (Ep-1 PD, Ep-1 PI, Ep-1 PL, Ep-1 PM, Ep- 1 PN) can be transmitted by mycotic contact to some strains of S.pneumoniae , Make its mycelial growth slow and abnormal branches. The results also show that: these weak pathogenicity S. sclerotiorum infection is strain-specific.