作者对干燥保存77d的人角膜内皮细胞用茜素红和台盼蓝联合染色后,六角形细胞边界隐约可见;核呈蓝色,圆形或卵圆形,其分布密度与新鲜角膜无区别.电镜观察,可见细胞轮廓,胞浆内细胞器清晰可见,且较丰富;核膜基本完整,仅核质淡,部分核皱缩.提示:干燥、脱水后保存的角膜内皮细胞,仍具有生命特征. The authors observed that the corneal endothelial cells stained with alizarin red and trypan blue stained for 77 days in a dry manner were vaguely visible on the borders of the hexagonal cells. The nuclei were blue, round or oval, and their distribution density was similar to fresh corneas. Electron microscopy showed that the cell contour was clear and the organelles were clearly visible and abundant in the cytoplasm. The nuclear membrane was basically intact, with only a weak nucleus and some nucleus shrinkage. Tip: The corneal endothelial cells preserved after dehydration and dehydration still have vital characteristics.