论文部分内容阅读
目的:检测胰腺癌组织中Smad4、p16的表达以及微血管密度(MVD)计数,探讨三者之间的关系。方法:应用免疫组化以及组织芯片技术检测82例胰腺癌组织和8例慢性胰腺炎组织中Smad4、p16和CD34的表达,通过观察CD34来测定MVD。结果:Smad4和p16的阳性率以及MVD在胰腺癌组织中分别为34.1%(28/82)、53.7%(44/82)、19.0±8.9,在慢性胰腺炎组织中分别为100.0%(8/8)、100.0%(8/8)、9.9±4.7;三者在两组间的差异均有统计学意义(P<0.05);Smad4、p16的表达与胰腺癌的分化程度部分有关,MVD与分化程度无关;胰腺癌Smad4和p16的表达均与MVD呈负相关(P<0.05),Smad4与p16的表达呈正相关(P<0.01)。结论:Smad4和p16的表达缺失可能在胰腺癌的发生中起到重要作用,作用方式可能与血管形成相关。
Objective: To detect the expression of Smad4, p16 in pancreatic cancer and the microvessel density (MVD) count to explore the relationship between the three. Methods: The expressions of Smad4, p16 and CD34 in 82 pancreatic cancer tissues and 8 chronic pancreatitis tissues were detected by immunohistochemistry and tissue microarray. The MVD was determined by observing CD34. Results: The positive rates of Smad4 and p16 and MVD in pancreatic cancer tissues were 34.1% (28/82), 53.7% (44/82) and 19.0 ± 8.9, respectively, and they were 100.0% (8 / 8), 100.0% (8/8) and 9.9 ± 4.7, respectively. The differences among the three groups were statistically significant (P <0.05). The expression of Smad4 and p16 was partly related to the degree of differentiation of pancreatic cancer. The expression of Smad4 and p16 in pancreatic cancer was negatively correlated with MVD (P <0.05), while the expression of Smad4 was positively correlated with p16 (P <0.01). Conclusion: The loss of Smad4 and p16 expression may play an important role in the pathogenesis of pancreatic cancer. The mode of action may be related to angiogenesis.