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目的研究鳄嘴花(CN)鲜榨汁及鲜药水提物对四氯化碳(CCl_4)造成的小鼠急性肝损伤的保护作用。方法 70只昆明种小鼠,雌雄各半,随机分成7组(n=10),正常组、模型组、联苯双酯组,低、高剂量鳄嘴花鲜榨汁组,低、高剂量鳄嘴花鲜药组,连续给药15d,末次给药2h后,除正常组腹腔注射花生油,其余各组均用0.1%CCl_4花生油溶液腹腔注射建小鼠急性肝损伤模型,禁食16h后取材测定血清丙氨酸氨基转移酶(ALT)、天冬氨酸转移酶(AST)、肝组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)及观察肝组织形态变化。结果与CCl_4模型组比较,各治疗组小鼠肝脏指数、血清ALT、AST水平不同程度降低(P<0.01);肝组织中SOD、GSH-Px水平不同程度升高(P<0.05或P<0.01),MDA水平不同程度降低(P<0.01);肝脏病理学检查显示,各治疗组均能不同程度的减轻肝细胞变性、坏死等。结论鳄嘴花对四氯化碳诱导的急性肝损伤具有一定的保护作用,其机制可能与抗氧化作用有关。
Objective To study the protective effects of freshly squeezed crayfish (CN) juice and fresh water extract on acute liver injury induced by carbon tetrachloride (CCl_4) in mice. Methods Seventy Kunming mice were randomly divided into 7 groups (n = 10), normal group, model group, bifendate group, low and high dose crocodile flower fresh juice group, low and high dose All rats were treated with 0.1% CCl 4 peanut oil solution intraperitoneally to establish acute hepatic injury model in mice. After fasting for 16 hours, the rats were sacrificed Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and observe the morphological changes of liver tissue. Results Compared with the CCl 4 model group, the liver index and serum ALT and AST levels of the mice in each treatment group decreased to different extents (P <0.01), while the levels of SOD and GSH-Px in the liver tissue increased to some extent (P <0.05 or P <0.01) ), MDA levels decreased to varying degrees (P <0.01); Liver pathology examination showed that each treatment group can reduce the degree of hepatocyte degeneration and necrosis to some extent. Conclusion Crocodilians flower has certain protective effect on carbon tetrachloride-induced acute liver injury, and its mechanism may be related to anti-oxidation.