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为了从水稻田这一特殊生境中分离到小单胞菌并研究其分布,在优化放线菌选择性分离方法的基础上对水稻田小单孢菌进行分离,分析比较了几种培养基和2种抑制剂对小单孢菌分离效果的影响。共分离出58株小单胞菌,对其中培养特征和形态特征明显不同的5个菌株进行了初步鉴定。结果表明:在YD和几丁质培养基上,小单孢菌出菌率比较高,小单孢菌占总放线菌的比例分别为63.6%和31.8%。在加入重铬酸钾的基础上加入2.0 g/L甲硝唑能明显抑制真菌和细菌的生长,对放线菌没有影响。基本解决了小单胞菌由于生长缓慢,容易被杂菌覆盖不易分出的问题。根据培养特征、形态特征、生理生化特征以及16S rDNA序列分析,5株分离菌株均为M icrom onospora sp.。
In order to isolate and study the distribution of small cell bacteria in this special habitat from paddy fields, Micromonospora sp. Was isolated on the basis of optimizing the selective isolation of actinomycetes. Several culture media and Effect of Two Kinds of Inhibitors on Micromonospora Isolation. A total of 58 strains of Chlamydomonas were isolated, and five strains with distinctly different characteristics of culture and morphological characteristics were identified. The results showed that in the YD and chitin medium, Micromonospora bacteria outbreak rate is relatively high, Micromonospora accounted for total actinomycetes were 63.6% and 31.8% respectively. Addition of 2.0 g / L metronidazole on the basis of potassium dichromate can obviously inhibit the growth of fungi and bacteria, and has no effect on actinomycetes. The basic solution to small cell growth due to slow, easy to be covered by the bacteria difficult to separate the problem. According to the culture characteristics, morphological characteristics, physiological and biochemical characteristics and 16S rDNA sequence analysis, all five isolates were M icrom onospora sp.