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现有结果表明64 DP(DNA结合蛋白)可能是一种急性期反应蛋白质。正常人每百毫升血清中含有约50mg的64 DP。目前,关于64 DP的生物学功能国内外尚无研究和报道。这方面的研究对阐明肿瘤及其它病理条件下血清64 DP含量增高的机制和意义有可能提供线索。 已知64 DP在体外具有和DNA结合的特性.它在体内的生理作用是否也和DNA有关呢?本实验首先分离纯化了人肝细胞染色质,用同位素标记的64 DP与染色质进行的结合实验及结合抑制实验表明64 DP能特异地与人染色质结合。进一步用~(125)I标记的兔抗64 DP抗体对人染色质作了固相放射免疫测定,结果定性地显示出染色质内可能含有64 DP。用兔抗64 DP抗体作为第一抗体,利用PAP免疫组织化学染色研究了16例正常肝组织切片,发现大多数切片(80％)中部分肝细胞核内有明显64DP显色颗粒。以上诸实验结果均提示细胞核内有64 DP存在,表明64 DP的生物学功能可能与它的DNA结合特性有关。
Existing results show that 64 DP (DNA binding protein) may be an acute phase response protein. Normal people contain about 50 mg of 64 DP per 100 ml serum. At present, there is no research or report on the biological function of 64 DP at home and abroad. This research may provide clues to elucidate the mechanism and significance of elevated serum 64 DP levels in tumors and other pathological conditions. It is known that 64 DP has the property of binding to DNA in vitro and whether its physiological role in the body is also related to DNA. In this experiment, chromatin from human hepatocyte was first isolated and purified, and the combination of isotope labeled 64 DP and chromatin Experiments and binding inhibition experiments show that 64 DP can specifically bind to human chromatin. Further, solid-phase radioimmunoassay of human chromatin with ~ (125) I labeled rabbit anti-64 DP antibody showed qualitatively that the chromatin may contain 64 DP. Using rabbit anti-64 DP antibody as the primary antibody, PAP immunohistochemical staining of 16 normal liver sections was performed, and it was found that in most of the sections (80%), there were obvious 64DP color particles in some of the liver cells. The results of these experiments suggest that 64 DP exists in the nucleus, indicating that the biological function of 64 DP may be related to its DNA binding properties.