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探讨温热、化疗药物、低渗液及其联合应用对小鼠腹水瘤细胞生长的防治作用,并探索结合热化疗药物达到最佳疗效的灌注液介质浓度。方法:以昆明小鼠腹水瘤为模型,观察43℃重蒸馏水(DDW)、37℃丝裂霉素(MMC)生理盐水溶液、43℃MMC不同浓度氯化钠溶液和43℃DDW MMC等不同处理方法对干扰腹水瘤细胞生长、杀伤作用及其毒性作用。结果:以上方法均能破坏小鼠腹水瘤细胞,减少瘤细胞数量,抑制腹水产生,延长小鼠生存时间,使S期肿瘤细胞百分率减少,瘤细胞DNA指数(DI)降低(P<0.05),其中以43℃DDW及MMC腹腔内灌注的方法疗效最佳。结论:低渗液结合热化疗药物行腹腔灌注是防治进展期消化道肿瘤患者发生腹膜种植转移的一种有效方法。
To investigate the preventive and therapeutic effects of warming, chemotherapeutic drugs, hypotonic fluid and their combination on the growth of mouse ascites tumor cells, and explore the combination of thermochemotherapy drugs to achieve the best perfusion medium concentration. Methods: Kunming mouse ascites tumor model was used to observe the effects of DDW at 43 ℃, MMC at 37 ℃, NaCl solution at 43 ℃ and DDW MMC at 43 ℃ Methods to interfere with ascites tumor cell growth, killing effect and its toxic effect. Results: All the above methods could destroy the ascites tumor cells, reduce the number of tumor cells, inhibit the production of ascites, prolong the survival time of mice, decrease the percentage of tumor cells in S phase and decrease the DNA index (DI) of tumor cells (P <0.05) Which 43 ℃ DDW and MMC intraperitoneal perfusion method is the best. CONCLUSION: Peritoneal perfusion is an effective method to prevent and treat peritoneal metastasis in patients with advanced gastrointestinal cancer with hypotonic solution combined with thermochemotherapy.