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本研究以粗皮桉无菌苗下胚轴为外植体,通过对不同激素组合的优化,研究不同激素处理对粗皮桉体胚发生的影响,初步建立了愈伤组织诱导及体胚发生最适合的激素浓度与种类。结果表明,0.005 mg/L TDZ和0.1 mg/L NAA激素组合诱导愈伤率最高,外植体愈伤组织诱导率达到97.5%,且该激素组合可以使体胚直接发生。而对体胚诱导效果最好的是1.0 mg/L 6-BA和0.1 mg/L NAA,诱导率为45.0%。本研究为粗皮桉体胚诱导提供借鉴与参考。
In this study, the hypocotyls of A. eucalyptus sterile seedlings were used as explants. The effects of different hormone treatments on somatic embryogenesis of Eucalyptus urophylla were studied by optimizing the combinations of different hormones. The optimal combination of callus induction and somatic embryogenesis was established Hormone concentration and type. The results showed that the combination of 0.005 mg / L TDZ and 0.1 mg / L NAA had the highest callus induction rate, the callus induction rate of explant reached 97.5%, and the combination of hormones could direct the somatic embryogenesis. The best induction of somatic embryos was 1.0 mg / L 6-BA and 0.1 mg / L NAA, the induction rate was 45.0%. This study provides a reference for the induction of somatic embryos in Eucalyptus.