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目的通过筛选建立裸小鼠人胃癌肝靶向高转移模型。方法采用原位移植技术,将组织学完整的MKN-45肿瘤组织块移植至裸小鼠胃壁上,按照胃原位移植→肝转移→皮下扩增→胃原位移植方法进行反复筛选,观察肿瘤原位移植成瘤率、侵袭和转移及形态学特征(光镜、电镜)。结果筛选出一个裸小鼠人胃癌肝靶向高转移模型,将其命名为MKN-45sci,已传至第5代。肿瘤的原位移植成瘤率、肝转移率和腹腔淋巴结转移率皆为100%,部分动物伴发肺、脾、膈等脏器转移及血性腹水。组织病理学和电镜观察结果证明其特征与原肿瘤相似。结论建立的裸小鼠人胃癌肝转移模型完整地再现了胃癌患者的临床过程,为探讨人胃癌肝转移的生物学机制和抗转移治疗提供了理想的实验动物模型
Objective To establish a liver-targeted high metastasis model of gastric cancer in nude mice by screening. Methods The orthotopically transplanted MKN-45 tumor tissue was transplanted into the stomach of nude mice by orthotopic transplantation. The tumors were retro-selected according to orthotopic transplantation of the liver → liver metastasis → subcutaneous expansion → orthotopic transplantation in situ. Tumorigenicity, invasion, metastasis and morphological features (light and electron microscopy) were transplanted in situ. Results A high-metastatic liver-targeted model of human gastric cancer was screened and named MKN-45sci. Tumor in situ implantation rate, liver metastasis and abdominal lymph node metastasis rates were 100%, some animals associated with lung, spleen, diaphragm and other organ metastases and bloody ascites. Histopathology and electron microscopy showed that the characteristics were similar to the original tumor. Conclusion The established model of human gastric cancer with liver metastasis completely reproduces the clinical course of gastric cancer patients and provides an ideal experimental animal model for exploring the biological mechanism and anti-metastasis treatment of liver metastasis of human gastric cancer