【摘 要】
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目的探讨三氧化二砷(ATO)对白血病K562细胞株基质金属蛋白酶-2、9(MMP-2、MMP-9)活性的影响.方法用0、0.05、0.40和3.20 μmol/L的ATO分别处理K562细胞,培养24、48、72 h后收
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目的探讨三氧化二砷(ATO)对白血病K562细胞株基质金属蛋白酶-2、9(MMP-2、MMP-9)活性的影响.方法用0、0.05、0.40和3.20 μmol/L的ATO分别处理K562细胞,培养24、48、72 h后收获细胞,对细胞上清进行明胶酶谱分析.结果①MMP-2、9在K562细胞均有表达,其活性条带在对照组以MMP-2(72 kd)最宽,MMP-9次之,MMP-2(62 kd)最窄.②MMP-2、9经0.05 μmol/L ATO处理72 h后差异具有显著性(P<0.05);经0.4、3.2 μ
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