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研究了二苯并吡喃类染料吡口罗红Y(PY)与核酸作用的共振光散射光谱,在pH11.5～12.0Tris-NaOH缓冲溶液中,1.75×10-5mol/L的PY溶液加入核酸后,体系的共振光散射增强,在364.0nm处,存在一共振光散射增强峰,其增强强度(△IRLS=I-I0)与核酸浓度呈线性关系,据此建立了一种测定纳克级核酸的简便灵敏的方法.对于fsDNA,方法的线性范围为27.0～625ng/mL,检出限为8.1ng/mL;对于ctD NA,方法的线性范围为39.0～500ng/mL,检出限为11.7ng/mL;对于yRNA,方法的线性范围为59.0～375ng/mL,检出限为17.7ng/mL.建立的方法已用于六种合成样品的测定,结果令人满意.
The resonance light scattering (DLS) spectra of dibenzopyran dye Pyrrolizidine Y (YPY) and nucleic acid were studied. In pH 11.5 ~ 12.0 Tris-NaOH buffer solution, 1.75 × 10-5 mol / L PY solution was added The results showed that there was a resonance light scattering enhancement peak at 364.0 nm and a linear relationship between the enhancement intensity (ΔIRLS = I-I0) and the nucleic acid concentration. Based on this, For the fsDNA, the linear range of the method is 27.0 ~ 625ng / mL, the detection limit is 8.1ng / mL; for ctD NA, the linear range of the method is 39.0 ~ 500ng / mL, the detection limit is The linear range of yRNA was 59.0 ~ 375ng / mL and the detection limit was 17.7ng / mL.The established method was applied to the determination of six synthetic samples with satisfactory results.