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目的探讨儿童钙代谢相关激素与雌激素受体(ER)及维生素D受体(VDR)基因多态性的关系。方法选取河南省某地140名8～12岁中国汉族健康儿童作为研究对象,抽取空腹外周血,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测ERα基因PvuⅡ,XbaⅠ以及VDR基因FokⅠ多态性;放免法测定血清骨钙素(OC)和降钙素(CT)浓度。结果携带ER PvuⅡ3种基因型儿童血清OC浓度分别为PP 5.82μg/L,Pp5.01μg/L,pp 6.21μg/L,差异有统计学意义(P<0.05),携带纯合pp基因型儿童血清OC浓度高于另外2组儿童;血清Ca、CT浓度在ER PvuⅡ各基因型间差异无统计学意义(P>0.05);携带VDR FokⅠ不同基因型儿童血清Ca浓度分别为ff2.71 mmol/L,Ff 2.39 mmol/L,FF 2.48 mmol/L,携带ff基因型儿童血清Ca浓度高于其余2种基因型儿童,差异有统计学意义(P<0.05);血清CT和OC浓度在FokⅠ各基因型差异无统计学意义(P>0.05);结论 ER PvuⅡ不同基因型可能影响血清OC浓度;血清钙浓度可能受VDR基因FokⅠ多态性的影响。
Objective To investigate the relationship between calcium-related hormones and estrogen receptor (ER) and vitamin D receptor (VDR) gene polymorphism in children. Methods A total of 140 healthy Han children aged 8-12 years old from a certain place in Henan Province were enrolled in this study. Fasting peripheral blood was collected and PCR-RFLP was used to detect the expression of ERα gene PvuⅡ, XbaⅠ And Fok Ⅰ polymorphism of VDR gene. Serum levels of osteocalcin (OC) and calcitonin (CT) were determined by radioimmunoassay. Results Serum OC levels in children with genotypes of ER PvuⅡ were PP 5.82μg / L, Pp 5.01μg / L and pp 6.21μg / L, respectively, with significant difference (P <0.05). Serum levels of homozygous pp genotype (P> 0.05). Serum Ca concentrations in children with different VDR FokⅠ genotypes were ff2.71 mmol / L , Ff 2.39 mmol / L and FF 2.48 mmol / L respectively. The serum Ca concentration in children with ff genotype was higher than those in the other two genotypes (P <0.05). The levels of serum CT and OC in FokⅠgenes (P> 0.05). CONCLUSION: Different genotypes of ER PvuⅡ may affect serum OC concentration. Serum calcium concentration may be affected by Fok Ⅰ polymorphism of VDR gene.