论文部分内容阅读
目的探讨组织蛋白酶D(Cathepsin D)在人淋巴瘤细胞株Raji中的表达水平变化与Raji细胞增殖和对阿霉素(Adriamycin,ADM)化疗敏感性的关系。方法实验将对数生长期Raji细胞分为5组:①空白对照组;②二甲基亚砜(DMSO)对照组;③胃酶抑素A(Pepstatin A)处理组;④ADM处理组;⑤Pepstatin A+ADM处理组。Western blot法检测ADM处理前后各组Raji细胞Cathepsin D表达水平的变化。四甲基偶氮唑蓝(MTT)法检测用Pepstatin A预处理后,Raji细胞对ADM敏感性的变化。结果Cathepsin D在Raji细胞中有表达,与空白对照组相比,Pepstatin A处理组和Pepstatin A+ADM处理组的Cathepsin D表达均有下降,两组的下降程度无统计学差异(P<0.05);而ADM处理组Raji细胞中Cathepsin D表达增高(P<0.05)。MTT结果显示,单用Pepstatin A处理,对Raji细胞增殖无影响,而ADM处理组及Pepstatin A+ADM处理组均明显抑制Raji细胞增殖,且Pepstatin A+ADM处理组的细胞增殖抑制率显著低于ADM处理组(P<0.05)。结论在ADM作用下,Raji细胞Cathepsin D表达上调,细胞增殖抑制率显著增高。据此推测Cathepsin D可能参与ADM介导的Raji细胞的凋亡,并能增强Raji细胞对ADM的化疗敏感性。
Objective To investigate the relationship between the expression of Cathepsin D in human Raji cell line Raji and the proliferation of Raji cells and chemosensitivity to Adriamycin (ADM). Methods The logarithmic growth phase Raji cells were divided into five groups: ① blank control group; ② dimethylsulfoxide (DMSO) control group; ③ pepstatin A treatment group; ④ADM treatment group; ⑤Pepstatin A + ADM treatment group. Western blot was used to detect the expression of Cathepsin D in Raji cells before and after ADM treatment. The sensitivity of Raji cells to ADM after pretreatment with Pepstatin A was detected by MTT assay. Results Cathepsin D was expressed in Raji cells. Compared with the blank control group, the expression of Cathepsin D in Pepstatin A group and Pepstatin A + ADM group was decreased, but there was no significant difference between the two groups (P <0.05) ; While the expression of Cathepsin D increased in Raji cells treated with ADM (P <0.05). The results of MTT showed that the single treatment with Pepstatin A had no effect on the proliferation of Raji cells. However, the proliferation of Raji cells was inhibited by ADM and Pepstatin A + ADM, and the inhibition rate of proliferation by Pepstatin A + ADM was significantly lower than ADM treatment group (P <0.05). Conclusions Under the action of ADM, the expression of Cathepsin D in Raji cells is up-regulated, and the inhibition rate of cell proliferation is significantly increased. It is speculated that Cathepsin D may be involved in ADM-mediated Raji cell apoptosis, and Raji cells to enhance chemosensitivity to ADM.