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目的:研究血清超敏C反应蛋白(hs-CRP)、白细胞介素-6(IL-6)和白细胞介素-10(IL-10)在糖尿病患者中的变化,及其对心血管并发症的预示作用。方法:收集99例糖尿病患者血清,其中胆固醇正常者68例,胆固醇升高者31例,及50例正常体检人员的血清。使用乳胶增强免疫透射比浊法检测hs-CRP,一步酶法检测胆固醇,同时采用ELISA双抗体夹心法测定血清IL-6、IL-10。结果:正常对照组血清hs-CRP、IL-6和IL-10浓度分别为(1.66±0.95)mg/L、(24±8)ng/L和(27±7)ng/L,胆固醇正常糖尿病组血清hs-CRP、IL-6和IL-10浓度分别为(2.77±1.19)mg/L、(25±10)ng/L和(28±8)ng/L,胆固醇升高糖尿病组血清hs-CRP、IL-6和IL-10浓度分别为(5.70±1.81)mg/L、(34±10)ng/L和(21±5)ng/L。与正常对照组、胆固醇正常糖尿病组相比,胆固醇升高糖尿病组hs-CRP、IL-6和IL-10表达水平差异均有统计学意义(分别为P<0.01、P<0.05、P<0.05)。与正常对照组比较,胆固醇正常糖尿病组hs-CRP高表达(P<0.05),而这两组间IL-6和IL-10表达水平差异无统计学意义(P>0.05)。结论:炎症因子hs-CRP在预测糖尿病患者并发冠心病中具有重要的临床意义。
Objective: To investigate the changes of serum hs-CRP, IL-6 and IL-10 in patients with diabetes mellitus, and their effects on cardiovascular complications The predictive role. Methods: Ninety-nine patients with diabetes mellitus were collected, including 68 normal cholesterol, 31 elevated cholesterol and 50 normal medical staff. Serum levels of IL-6 and IL-10 were detected by latex enzyme-enhanced immunoturbidimetric assay for hs-CRP and cholesterol by one-step enzymatic assay. RESULTS: The serum concentrations of hs-CRP, IL-6 and IL-10 in the normal control group were (1.66 ± 0.95) mg / L, (24 ± 8) ng / L and (27 ± 7) ng / The serum concentrations of hs-CRP, IL-6 and IL-10 in the serum of diabetic patients with elevated cholesterol (2.77 ± 1.19 mg / L, 25 ± 10 ng / L and 28 ± 8 ng / The concentrations of -CRP, IL-6 and IL-10 were (5.70 ± 1.81) mg / L, (34 ± 10) ng / L and (21 ± 5) ng / L, respectively. The levels of hs-CRP, IL-6 and IL-10 in cholesterol-increased diabetic group were significantly lower than those in normal control group and normal cholesterol group (P <0.01, P <0.05, P <0.05 ). Compared with the normal control group, hs-CRP in normal cholesterol group was significantly higher than that in normal control group (P <0.05), while there was no significant difference between the two groups in the expression of IL-6 and IL-10 (P> 0.05). Conclusion: The inflammatory factor hs-CRP in the prediction of diabetic patients with coronary heart disease has an important clinical significance.