论文部分内容阅读
目的 :用TLC法测定板兰根、大青叶及其板兰根冲剂中靛苷含量。方法 :将板兰根、大青叶及板兰根冲剂中所含靛苷水解后与吲哚醌缩合生成靛玉红 ,再用TLC进行测定。双波长反射法锯齿扫描 ,λs=5 40nm ,λR=70 0nm ;狭缝宽度 :0 2mm× 0 2mm ;灵敏度 :中 ;线性化器 :SX =3;展开剂 :氯仿 -丙酮 (12∶0 5 )。结果 :靛玉红标准曲线线性范围 0 0 8～ 0 2 4μg·mL-1;回归方程 :Y =2 49 0 3+ 382 0 4 6 1X ,r =0 9999;平均回收率 97 75 % (n=5 )。结论 :方法简便 ,准确、易行 ,可作为板兰根、大青叶及其制剂的质量控制方法。
Objective : To determine the glycoside content of Banlangen, Daqingye and its Banlangen granules by TLC. Methods: The indole glycoside contained in Banlangen, Daqingye and Banlangen granules was hydrolyzed and then condensed with hydrazine to form indirubin, which was then measured by TLC. Double-wavelength reflection method sawtooth scanning, λs=5 40nm, λR=70 0nm; slit width: 0 2mm× 0 2mm; sensitivity: medium; linearizer: SX =3; developing agent: chloroform-acetone (12:0) ). Results: The linear range of indirubin standard curve was 0 0 8～0 2 4μg·mL-1; the regression equation was: Y =2 49 0 3+ 382 0 4 6 1X ,r =0 9999; the average recovery rate was 97 75 % (n =5 ). Conclusion: The method is simple, accurate, and easy to use. It can be used as a quality control method for Banlangen, Daqingye and its preparations.