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目的 :探索核因子κB(NF κB)在肝癌的表达变化 ,以及与病理分期和抗凋亡基因Bcl 2表达的相关性 .方法 :采用免疫组织化学方法 ,检测NF κB在 4 0例肝细胞肝癌及 1 5例正常肝组织中的表达变化及抗凋亡基因Bcl 2的相应表达 .结果 :NF κB在肝癌组和正常肝组织组的阳性表达率分别为72 %、1 3% ,两者有显著性差异 (P <0 .0 1 ) ,而强阳性表达率分别 30 %和 7% ,Bcl 2在肝癌组和正常肝组织组的阳性表达率分别为 4 0 %和 7% ,两者有显著性差异 (P <0 .0 5 ) ,而强阳性表达率分别为 1 7%和 0 .NF κB的阳性表达在病理分级间有显著性差异 (P <0 .0 1 )且与Bcl 2的阳性表达有显著相关(r =0 .389,P =0 .0 1 3) .结论 :NF κB异常表达与肝癌的发生有关 ,并在一定程度上可以解释肝癌对化疗的抵抗作用 .NF κB与Bcl 2高度相关 ,表明NF κB可以通过对下游抗凋亡基因表达的调控 ,参与了肝癌的抗凋亡过程
Objective: To explore the relationship between the expression of NF-κB and the expression of Bcl-2 in hepatocellular carcinoma.Methods: Immunohistochemistry was used to detect the expression of NF-κB in 40 hepatocellular carcinoma And the expression of Bcl 2 in 15 normal liver tissues.Results: The positive rates of NF κB in hepatocellular carcinoma and normal liver tissues were 72% and 13% respectively (P <0.01), while the strong positive rates were 30% and 7% respectively. The positive rate of Bcl-2 in HCC and normal liver tissues was 40% and 7%, respectively (P <0.05), while the strong positive expression rate was 17% and 0. The positive expression of NFκB in the pathological grade was significantly different (P <0.01) and the positive correlation with Bcl 2 (R = 0.389, P = 0.013) .Conclusion: The abnormal expression of NF-κB is associated with the development of hepatocellular carcinoma and to a certain extent, it can explain the resistance of hepatocellular carcinoma to chemotherapy.NF κB It is highly correlated with Bcl 2, indicating that NF κB is involved in the anti-apoptotic process of hepatocarcinoma through the regulation of downstream anti-apoptotic gene expression