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河北杨(Populus hopeiensis)又名椴杨。是西北地区主要的造林树种之一。它发根能力差,繁殖困难。我们于1981年开始在刘榕副教授指导下进行河北杨茎尖离体培养的研究,现已获得完整植株并移栽成活,成活率高,具体操作如下: 本试验取材于河北杨的二年生休眠芽,或当年生幼嫩腋芽。切取枝条后用0.2%蔗糖蒸馏水溶液水培一个星期左右,以增加外植体的细胞液浓度,减少污染率。
Populus hopeiensis is also known as linden poplar. It is one of the main afforestation species in Northwest China. It is poor hair root, breeding difficulties. We started in 1981 under the guidance of Associate Professor Liu Rong Tsang Tsai tip culture in vitro studies have now been completed and transplanted plant survival rate, the survival rate is high, the specific operation is as follows: This experiment was based on Hebei Province biennial dormancy buds , Or when young and young axillary buds. After cutting the branches with 0.2% sucrose distilled water solution for about a week hydroponics, in order to increase the concentration of explant liquid, reduce the pollution rate.