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GTP结合蛋白是真核生物中一类信号开关分子,通过与GTP结合的活化状态和与GDP结合的非活化状态,参与调控多种信号传导通路和物质代谢。近年来,许多研究发现小G蛋白家族成员在植物细胞极性生长的生物学过程中具有非常重要的作用。为了研究GTP结合蛋白在小麦叶片表皮细胞极性分化中的作用,我们从小麦幼叶基部居间分生组织中克隆了一个尚未报道的小麦GTP结合蛋白基因,命名为Ta Rac3。为进一步验证该基因在表皮细胞发育过程中的生物学功能,我们利用生物信息学方法对该基因编码蛋白的保守结构域中的活性位点和羧基端多元基团区域的亚细胞定位信号进行了注释和分析,并进行了聚类分析。我们还构建了Ta Rac3基因的植物表达载体,并转入农杆菌细胞,为后期转化拟南芥进行生物学功能验证奠定了基础。
GTP-binding proteins are a class of signaling molecules in eukaryotes that participate in the regulation of a variety of signaling pathways and substance metabolism through the activation of GTP binding and the non-activation of GDP binding. In recent years, many studies have found that small G protein family members play a very important role in the biological process of polar growth of plant cells. In order to study the role of GTP-binding protein in the polarity differentiation of wheat leaf epidermis cells, we cloned an unreported wheat GTP-binding protein gene named Ta Rac3 from the meristematic meristem of young leaves. To further verify the biological function of this gene in epidermal cell development, we used bioinformatics methods to perform subcellular localization of the active site and carboxyl-terminal polybasic region in the conserved domain of the gene Annotation and analysis, and carried out cluster analysis. We also constructed the plant expression vector of Ta Rac3 gene and transferred it into Agrobacterium cells, which laid the foundation for the biological function verification of Arabidopsis thaliana transformed in the later stage.