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In the present study, the radioreceptor binding method was used to determine the changes of IP_3 content in rat brain and dorsal spinal cord of high frequency (100 Hz) electroacupnncture (EA) analgesia and EA tolerance rat. The control levels of IP_3 in rat brain (less cerebellum and cortex) and dorsal spinal cord were 6.3±0.78 pmol/mg protein and 3.4±0.60 pmol/mg protein, respectively. The results showed that IP_3 in brain increased gradually within 45 min after stimulation in EA analgesia rat. Meanwhile, the dorsal spinal cord IP_3 content decreased significantly 15 min, 30 min after EA stimulation and recovered to control level 45 min after EA stimulation. In EA tolerance rat, IP_3 contents markedly increased in brain. And IP_3 content in the spinal cord also increased dramatically within 30 min, but decreased rapidly to control level 45 min after EA stimulation. The IP_3 level in EA tolerance rat brain and spinal cord was much higher than that in EA analgesia rat (P<0.01). The studies first reporte
In the present study, the radioreceptor binding method was used to determine the changes of IP_3 content in rat brain and dorsal spinal cord of high frequency (100 Hz) electroacupnncture (EA) analgesia and EA tolerance rat. The control levels of IP_3 in rat brain (less cerebellum and cortex) and dorsal spinal cord were 6.3 ± 0.78 pmol / mg protein and 3.4 ± 0.60 pmol / mg protein, respectively. The results showed that IP_3 in brain increased gradually within 45 min after stimulation in EA analgesia rat. Meanwhile, The dorsal spinal cord IP 3 content decreased significantly for 15 min, 30 min after EA stimulation and recovered to control level 45 min after EA stimulation. In EA tolerance rat, IP 3 contents markedly increased in brain. And IP 3 content in the spinal cord also increased dramatically within 30 min, but decreased rapidly to control level 45 min after EA stimulation. The IP_3 level in EA tolerance rat brain and spinal cord was much higher than that in EA analgesia rat (P <0.01). The st udies first reporte