以观音莲的顶芽或侧芽为外植体进行离体培养 ,结果显示 6 -BA对观音莲丛生芽的诱导和增殖有促进作用 ,在MS +6 -BA 5mg/L的培养基上培养 30d ,增殖率为 4 1.丛生芽切割成小苗亦可增殖 NAA对增殖和生根均无影响 ,小苗在增殖培养基上即可生根 在 2 5～ 30℃的气温下 ,观音莲试管苗的移栽成活率可达 90 %以上 Guanyin lotus buds or lateral buds were used as explants for in vitro culture. The results showed that 6-BA promoted the induction and proliferation of clustered buds of Guanyin, and cultured on MS +6-BA 5mg / L medium for 30 days , The proliferation rate of 4 1. Cong Sheng buds can be cut into small seedlings can also proliferate NAA proliferation and rooting had no effect on the proliferation medium in the seedlings can be rooted at temperatures of 25 ~ 30 ℃, Guanyinglian transplanted in vitro Survival rate of up to 90%