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目的 探讨慢性低氧大鼠血清中胰岛素样生长因子 1(IGF 1)浓度、肺小动脉壁IGF 1多肽表达的变化及其与低氧性肺动脉高压 (HPH)之间的关系。方法 用放射免疫分析法检测大鼠血清中IGF 1浓度 ;用免疫组织化学染色法观察肺小动脉壁IGF 1多肽的表达 ;用图像分析技术观察肺小动脉的形态改变和IGF 1多肽的表达强度。结果 低氧性肺动脉高压大鼠管壁增厚 ,管腔狭窄 ,反映管壁增厚的两个指标MT %和MA %均显著高于正常对照组 (P <0 0 5 ) ;灰度扫描示低氧大鼠肺小动脉壁的阳性程度显著大于正常对照组 (P <0 0 1)。低氧大鼠血清IGF 1水平明显高于正常对照组 (P <0 0 5 ) ;大鼠血清IGF 1水平与平均肺动脉压 (mPAP)呈显著正相关 (r =0 745 ,P =0 0 0 2 )。结论 在低氧性肺血管重建的发生机制中 ,IGF 1不仅以自分泌和旁分泌的方式刺激血管平滑肌细胞增生肥厚 ,而且还存在内分泌机制。
Objective To investigate the changes of serum IGF-1, IGF1 in the pulmonary arterioles and its relationship with hypoxic pulmonary hypertension (HPH) in chronic hypoxia rats. Methods The serum IGF1 concentration was measured by radioimmunoassay. The expression of IGF1 polypeptide in pulmonary arteriole was observed by immunohistochemical staining. The morphological changes of pulmonary arterioles and the expression of IGF1 polypeptide were observed by image analysis . Results Hypoxic pulmonary hypertensive rats had thicker thoracic wall and narrowed lumen, and the MT% and MA% of the two indicators of wall thickening were significantly higher than those of normal control group (P <0.05) The positive degree of pulmonary arterioles in hypoxic rats was significantly greater than that in normal controls (P <0.01). The level of serum IGF1 in hypoxic rats was significantly higher than that in normal controls (P <0.05). The level of IGF1 in serum was positively correlated with mean pulmonary arterial pressure (mPAP) (r = 0 745, P = 0 0 0 2 ). Conclusion In the pathogenesis of hypoxic pulmonary vascular remodeling, IGF 1 not only stimulates the proliferation and proliferation of vascular smooth muscle cells in an autocrine and paracrine manner, but also has an endocrine mechanism.