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本研究从几种热带高蛋白发酵食品中分离蛋白酶高产菌株，在所分离的２０株菌株中，有三株在蔗糖废蜜培养基试管培养中即超过５单位／ｍＬ（即微克单位的１０００单位／ｍＬ），比１９９０年Ｍａｒｇａｒｉｔａ分离到的枯草杆菌ＢＩＯＴＥＣＨ１１３的１．３单位／ｍＬ和１９９３年Ｍｏｏｎ分离到的坚强芽胞杆菌ＮＲＳ的１．２单位／ｍＬ高三倍多．初步鉴定为芽胞杆菌属第一群的Ｂ亚群．其中一株用蔗糖废蜜培养基在３Ｌ搅拌通气发酵罐中发酵于７ｈ内产生１．４单位／ｍＬ的蛋白酶活性．通过补充大豆饼粉和蔗糖废蜜，培养到３５ｈ细胞数量和蛋白酶活力分别达到１０１０／ｍＬ和７６单位／ｍＬ（即我国微克单位的１３７７０单位／ｍＬ）．比我国生产用菌株枯草芽胞杆菌ＡＳ１．３９８和地衣芽胞杆菌２７０９的高近一倍．蛋白酶生产的活力随通气和搅拌速度的减小而减小，随通气和搅拌速度的增加而增加．所产蛋白酶的最佳作用ｐＨ为８．５．且在此ｐＨ８．５的条件下是稳定的．
In this study, several strains of high protein fermentative protein isolated from tropical high protein fermentation strains, of the 20 strains isolated, three in sucrose waste honey medium tube culture that is more than 5 units / mL (ie, 1000 units of microgram units / mL), more than three times higher than 1.3 units / mL of B. subtilis BIOTECH113 isolated in Margarita in 1990 and 1.2 units / mL of B. firmus NRS isolated in 1993 Moon. Preliminary identification of Bacillus is the first group of B subgroups. One of them was sucrose waste honey culture medium in 3L stirred aeration fermenter fermentation within 7h produce 1.4 units / mL of protease activity. The cell number and protease activity reached 1010 / mL and 76 units / mL (that is, 13770 units / mL in our country) in 35h after supplementing soybean meal and sucrose waste honey. Than our production strains Bacillus subtilis AS1.398 and Bacillus licheniformis 2709 nearly doubled. The vitality of protease production decreases with decreasing aeration and agitation speed and increases with aeration and agitation speed. The optimum pH of the protease produced was 8.5. And is stable at pH 8.5.