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目的:探讨冷冻胚胎周期不同年龄段患者如何制定移植策略。方法:回顾性队列研究分析浙江省宁波市妇女儿童医院生殖中心取卵后全胚冷冻并于2018年1月至2020年4月期间首次行冻融胚胎移植患者的临床资料，根据患者的年龄分为三组，即≤30周岁组（1150个周期）、30~35周岁组（932个周期）及>35周岁组（405个周期）；在每个年龄组内，再根据移植胚胎级别及移植数目分为第3日单卵裂期胚胎移植（single cleavage stage embryo transplant， SET3） 亚组、第5日单囊胚移植 （single 5th day blastocyst transplant， SET5） 亚组、第 6 日单囊胚移植 （single 6th day blastocyst transplant， SET6） 亚组、第3日双卵裂期胚胎移植（double cleavage stage embryo transplant， DET3）亚组、第 5 日双囊胚移植 （double 5th day blastocyst transplant， DET5） 亚组、第6日双囊胚移植 （double 6th day blastocyst transplant， DET6） 亚组。所有患者均随访至2020年6月30日，比较同一年龄组内各亚组的临床结局。结果:①≤30周岁组： SET5亚组临床妊娠率、持续妊娠率及活产率分别为66.17%（221/334）、60.48%（202/334）及35.93%（120/334），分别高于SET3亚组［41.18%（28/68）、36.76%（25/68）、25.00%（17/68）， n P<0.001、n P=0.001、n P=0.045］及SET6亚组［54.42%（80/147）、43.54%（64/147）、27.21%（40/147）， n P<0.001、n P=0.001、n P<0.001］； DET5亚组多胎率［47.80%（76/159）］高于DET6亚组［29.49%（23/78）］（n P=0.007）。 ②30~35周岁组：SET5亚组临床妊娠率、持续妊娠率及活产率分别为63.59%（138/217）、55.30%（120/217）及29.95%（65/217），分别高于SET3亚组［30.65%（19/62）、27.42%（17/62）、14.52%（9/62），n P<0.001、n P<0.001、n P=0.015］； DET5亚组多胎率［56.86%（58/102）］高于DET3亚组［32.54%（41/126）］及DET6亚组［28.57%（16/56）］，差异均有统计学意义（n P<0.001及n P=0.001）。③35周岁以上组： SET5亚组临床妊娠率、持续妊娠率及活产率［53.45%（31/58）、39.66%（23/58）、20.69%（12/58）］与SET6亚组［43.75%（21/48）、35.42%（17/48）、16.76%（8/48）］比较，差异均无统计学意义（n P=0.320、n P=0.655、n P=0.583），高于SET3亚组［16.98%（9/53）、15.09%（8/53）、7.55%（4/53）， n P<0.010、n P=0.004、n P=0.049］； DET5亚组临床妊娠率及持续妊娠率［66.67%（26/39），53.85%（21/39）］均高于DET3亚组［33.89%（61/180），27.22%（49/180）］，差异均有统计学意义（n P35 years old group (405 cycles). In each age group, according to the grade and number of transferred embryos, they were divided into the single cleavage stage embryo transplant (SET3) subgroup, the single 5th day blastocyst transplant (SET5) subgroup, the single 6th day blastocyst transplant (SET6) subgroup, the double cleavage stage embryos transplant (DET3) subgroup, the double 5th day blastocyst transplant (DET5) subgroup and the double 6th day blastocyst transplant (DET6) subgroup. All patients were followed up until June 30, 2020. The clinical outcomes of each subgroups within the same age group were compared.Results:1) In ≤30 years old group: the clinical pregnancy rate, the persistent pregnancy rate and the live birth rate in SET5 subgroup were 66.17% (221/334), 60.48% (202/334), 35.93% (120/334), respectively, which were higher than those in SET3 subgroup [41.18% (28/68), 36.76% (25/68), 25.00% (17/68); n P<0.001,n P=0.001,n P=0.045] and that in SET6 subgroup [54.42% (80/147), 43.54% (64/147), 27.21% (40/147); n P<0.001,n P=0.001, n P<0.001]. The multiple rate in DET5 subgroup was 47.80% (76/159), which was higher than that in DET6 subgroup [29.49% (23/78),n P=0.007]. 2) In 30-35 years old group: the clinical pregnancy rate, the persistent pregnancy rate and the live birth rate in SET5 subgroup were 63.59% (138/217), 55.30% (120/217) and 29.95% (65/217), which were higher than those in SET3 subgroup [30.65% (19/62), 27.42% (17/62),14.52% (9/62);n P<0.001,n P<0.001,n P=0.015]. The multiple rate in DET5 subgroup was 56.86% (58/102), which was higher than that in DET3 subgroup [32.54% (41/126)] and DET6 subgroup [28.57% (16/56)], and the differences were statistically significant (n P35 years old group: the clinical pregnancy rate, the sustained pregnancy rate and the live birth rate in DET5 subgroup were 53.45% (31/58), 39.66% (23/58), 20.69% (12/58), compared with those in SET6 subgroup [43.75% (21/48), 35.42% (17/48), 16.76% (8/48)], the differences were not statistically significant ( n P=0.320,n P=0.655,n P=0.583), while compared with SET3 subgroup [16.98% (9/53), 15.09% (8/53), 7.55% (4/53)], the differences were statistically significant (n P<0.010,n P=0.004, n P=0.049). The clinical pregnancy rate and the sustained pregnancy rate in DET5 subgroup were 66.67% (26/39), 53.85% (21/39), which were higher than those in DET3 subgroup [33.89% (61/180), 27.22% (49/180)], and the differences were statistically significant (n P<0.001n , P=0.001). The clinical pregnancy rate in DET5 subgroup was higher than that in DET6 subgroup [51.85% (14/27)], and the difference was statistically significant (n P=0.001)n .Conclusion:For patients of all ages, single embryo transfer should be selected, and avoid the transfer of two 5th day blastocysts as far as possible. The 5th day single blastocyst is preferred for embryo transfer.