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目的 探讨神经生长因子 (NGF)改善衰老性记忆障碍的机制。方法 采用开场行为和一次性被动回避反应模型 ,观察海马内微量注射NGF对衰老小鼠自发活动和记忆巩固过程的影响 ,同时用Ca2 +荧光探针Fura 2 /AM和AR CM MIC阳离子测定系统测定海马突触体游离钙水平 ,以3H Leu为标记物测定海马突触体总蛋白的合成量。结果 NGF使衰老小鼠在新异环境中的自发活动和探究行为明显增多 ,并显著延长电击后 2 4h的步入潜伏期(STL) ;NGF显著降低衰老小鼠海马突触体内的高钙水平 ,并使海马突触体蛋白质的3H Leu参入量显著增加。结论 NGF改善衰老性记忆障碍与其降低海马突触体内高钙水平 ,并由此促进海马突触体蛋白质的合成有关。
Objective To investigate the mechanism of nerve growth factor (NGF) improving aging memory impairment. Methods The opening behavior and one-time passive avoidance response model were used to observe the effect of microinjection of NGF into the hippocampus on spontaneous activity and memory consolidation in senile mice. The Ca 2+ fluorescence probe Fura 2 / AM and AR CM MIC cation determination system Hippocampal synaptosome free calcium levels, 3H-Leu as a marker for the determination of total hippocampal synaptosome synthesis. Results NGF significantly increased the spontaneous and exploratory behaviors of aged mice in the novel environment and prolonged the onset latency (STL) of 24 h after shock. NGF significantly decreased the level of high calcium in the hippocampal synapse of aging mice, And the amount of 3H Leu incorporation in hippocampal synaptosomes increased significantly. Conclusions NGF can improve aging memory impairment by decreasing hippocampal synaptic hypercalcemia and thus promoting synaptic protein synthesis in the hippocampus.