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Six Inner Mongolian semifine-wool wethers (1.5 years old,29-31 kg BW) fitted with a ruminal and a duodenum cannula were used to study the effects of increasing production rate of ruminal acetate and butyrate on their absorption and passage in alimentary tract by simulating continuous feeding and pulsecontinuous infusion technology.The sheep were divided into two groups randomly and fed the same basal diet,one group was for acetate measuring and the other group was for butyrate measuring.Diet was formulated according to maintain requirement of Inner Mongolian sheep and consisted of hay 69.64%,corn 18.11%,soybean meal 15.57%,wheat bran 5.57%,and premix 1.11%,the diet contained DM 92.34%,CP9.74%,ME 8.47 MJ/kg,Ca 0.31%,P 0.21%(dry matter basis).Three infusion levels of acetate and butyrate were designed to reach 2.5,3.0 and 4.0 multiple on the basis of basal production rate.The rumen and duodenum fluid samples were collected for measuring pH,Co-EDTA,acetate and butyrate concentration.