论文部分内容阅读
目的:探讨纽卡斯尔病毒疫苗(ATV-NDV)和白细胞介素-12(IL-12)对3AO裸鼠皮下移植瘤的影响及其作用机制。方法:建立3AO裸鼠移植瘤模型,随机分为4组,每组10只。Ⅰ组注射ATV-NDV(0.2mL);Ⅱ组注射IL-12(100ng/kg);Ⅲ组注射IL-12(100ng/kg)加ATV-NDV(0.2mL);Ⅳ组为对照组,腹腔注射生理盐水0.2mL;观察各组裸鼠成瘤率、生存期和生存延长率及肿瘤生长曲线。流式细胞术(FCM)观察裸鼠移植瘤细胞凋亡率及细胞周期,双标记计数NK细胞比例;称取裸鼠脾脏质量;移植瘤瘤体及各组裸鼠肝、脾、肾行组织病理学变化检查。结果:治疗组在荷瘤裸鼠生长延长率,裸鼠移植瘤细胞凋亡率,NK细胞群计数,裸鼠脾脏重量方面明显高于对照组,Ⅲ组高于Ⅰ组和Ⅱ组。组织病理学检查各治疗组中均可见有肿瘤坏死,肿瘤内及周围有明显的淋巴细胞浸润。结论:ATV-NDV和IL-12对人卵巢癌裸鼠皮下移植瘤具有明显的抑制作用。IL-12可增强ATV-NDV对卵巢癌的抑制作用。
Objective: To investigate the effects of Newcastle virus vaccine (ATV-NDV) and interleukin-12 (IL-12) on subcutaneous xenografts in 3AO nude mice and its mechanism. Methods: 3AO nude mice transplantation model was established and randomly divided into 4 groups with 10 in each group. GroupⅠwas injected with ATV-NDV (0.2mL); GroupⅡwas injected with IL-12 (100ng / kg); GroupⅢwas injected with IL-12 (100ng / kg) The rats were injected with 0.2 mL of normal saline. The tumor formation rate, survival rate, survival rate and tumor growth curve of nude mice in each group were observed. The apoptotic rate and cell cycle of nude mice were observed by flow cytometry (FCM), and the proportion of NK cells was counted by dual-labeled method. The spleen mass of nude mice was weighed; Pathological changes check. Results: The growth of tumor-bearing mice in nude mice, the apoptosis rate of nude mice, NK cell count and nude mice spleen weight were significantly higher than those in control group. Histopathological examination showed that all the treatment groups showed tumor necrosis with obvious lymphocytic infiltration in and around the tumor. Conclusion: ATV-NDV and IL-12 have significant inhibitory effects on subcutaneous xenografts of human ovarian cancer in nude mice. IL-12 enhances the inhibitory effect of ATV-NDV on ovarian cancer.