作者取12例食管癌手术标本,将癌组织及正常粘膜部分分别用胰蛋白酶混合液消化,获取分散细胞、检测y-GT活性。细胞来自癌组织,58％(7/12)的标本,表现有у-GT活性;细胞来自正常粘膜部分,100％(12/12)标本,都检测到у-GT阳性细胞。另取11例浸润癌手术标本,取肿瘤区、肿瘤相邻区以及远离肿瘤区组织作切片,进行y-GT及H-E染色。在肿瘤区y-GT阳性例数为8/11,相邻肿瘤区为11/11, The authors took 12 cases of surgical specimens of esophageal cancer, the cancer tissue and normal mucosa were digested with a trypsin mixture, to obtain scattered cells and detect y-GT activity. The cells were from cancerous tissues, 58% (7/12) of the specimens showed у-GT activity; the cells were from normal mucosa, and 100% (12/12) specimens detected у-GT-positive cells. Another 11 invasive cancer surgical specimens were taken from the tumor area, tumor adjacent area, and tissue away from the tumor area for sectioning, and y-GT and H-E staining was performed. The positive number of y-GT in the tumor area was 8/11, and the adjacent tumor area was 11/11.