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研究了雌黄纳米粒的制备方法及其抗肿瘤作用.采用化学方法制备A s2S3纳米粒,通过透射电镜、X射线能谱(EDS)对A s2S3纳米粒进行分析表征,以形态学、MTT法和流式细胞术体外研究不同浓度的A s2S3纳米粒对肝癌SMMC-7721细胞生长的影响,并同时与传统剂型的A s2S3进行比较.分析结果显示:实验制备的A s2S3纳米粒平均直径约为80nm,电镜下呈圆形,大小较一致,分散性较好,EDS证实其为A s2S3,无其他成分;A s2S3纳米粒对SMMC-7721细胞有明显的生长抑制和凋亡诱导作用,且呈浓度和时间依耐性,其细胞生长抑制率和凋亡诱导率明显高于相同浓度传统剂型的A s2S3处理组(p<0.001).表明化学法可以制备A s2S3纳米粒;与传统剂型的A s2S3相比,A s2S3纳米粒有更强的抗肿瘤作用.
The preparation method of estrogen nanoparticles and its anti-tumor effect were studied.A s2S3 nanoparticles were prepared by chemical method and characterized by transmission electron microscopy and energy dispersive X-ray spectroscopy (EDS). Morphology, MTT method and Flow cytometry was used to study the effect of different concentrations of A s2S3 nanoparticles on the growth of SMMC-7721 hepatocellular carcinoma cells in vitro and at the same time compared with the traditional formulation of A s2S3.The results showed that the average diameter of A s2S3 nanoparticles prepared in experiment was about 80 nm , Electron microscopy round, the size is more consistent, better dispersion, EDS confirmed that it is A s2S3, no other components; A s2S3 nanoparticles on SMMC-7721 cells were significantly inhibited by growth and apoptosis induced by concentration And time-dependent, the cell growth inhibition rate and apoptosis induction rate were significantly higher than the A s2S3 treatment group (p <0.001) of the same dosage of the traditional dosage form, indicating that A s2S3 nanoparticles can be prepared by chemical method; A s2S3 nanoparticles have a stronger anti-tumor effect.