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背景:去卵巢动物多用于女性骨质疏松的研究模型,但少有研究去卵巢对动物能量平衡的影响。以前研究大豆异黄酮可降低高脂饲料引起的大鼠高脂血症,大豆异黄酮对去卵巢动物的能量代谢如何?目的:探讨大豆提取物及其有效成分对去卵巢大鼠能量代谢的药理作用,为2型糖尿病、脂代谢紊乱、高血压和冠状动脉粥样硬化性心脏病的一级康复预防提供理论依据。设计:以实验动物为研究对象的随机对照实验研究。单位:一所大学的细胞与生物化学实验室。材料:Wistar大鼠90只(SFP级,合格证号:scxk11-00-006)随机分成正常组、假手术组、模型组、雌激素组、高剂量大豆黄酮组、低剂量大豆黄酮组、高剂量大豆提取物组、低剂量大豆提取物组、大豆多糖组,每组10只。干预:除假手术组与正常组外,其余各组均切除双侧卵巢。术后1周开始每周测量一次体质量、日进食量。6周后处死动物,计算饲料转化率,测量身长,计算体质量系数,分离腹部脂肪并称重。主要观察指标:①大豆提取物对去卵巢大鼠腹部脂肪堆积的作用。②大豆提取物对去卵巢大鼠进食量的影响。③大豆提取物对去卵巢大鼠饲料转化率的作用。结果:切除卵巢后动物进食量、体质量与体质量系数增加、饲料转化率加大,腹部脂肪堆积。雌激素组、大豆提取物组及大豆黄酮组均不同程度减弱去卵巢引起的能量代谢改变,但大豆多糖无作用。结论:去卵巢大鼠可作为更年期妇女肥胖的动物模型,大豆提取物有减弱去卵巢引起的体质量增加、进食和饲料转化率增加等病理改变,其有效成分为黄酮类化合物。
BACKGROUND: Ovariectomized animals are mostly used in research models of osteoporosis in women, but few studies have investigated the effect of ovariectomy on animal energy balance. Previous studies on soy isoflavones can reduce hyperlipidemia in rats with high-fat diet. How does soybean isoflavones affect energy metabolism in ovariectomized animals? Objective: To investigate the pharmacological effects of soybean extract and its active ingredients on energy metabolism in ovariectomized rats. The role of providing theoretical basis for the first-class rehabilitation and prevention of type 2 diabetes, lipid metabolism disorders, hypertension and coronary atherosclerotic heart disease. Design: A randomized, controlled experimental study of experimental animals. Unit: Cell and biochemistry laboratory at a university. MATERIALS: Ninety Wistar rats (SFP grade, certificate number: scxk11-00-006) were randomly divided into normal group, sham group, model group, estrogen group, high-dose daidzein group, low-dose daidzein group, and high-dose group. Dose soy extract group, low-dose soybean extract group, soybean polysaccharide group, 10 in each group. Intervention: Except for the sham group and the normal group, all other groups were excised with bilateral ovaries. One week after surgery, body weight and daily intake were measured once a week. After 6 weeks, the animals were sacrificed, the feed conversion rate was calculated, the body length was measured, the body mass coefficient was calculated, and abdominal fat was separated and weighed. MAIN OUTCOME MEASURES: The effect of soybean extract on abdominal fat accumulation in ovariectomized rats. 2 The effect of soybean extract on food intake in ovariectomized rats. 3 The effect of soybean extract on the feed conversion rate of ovariectomized rats. RESULTS: After the ovariectomy, the animal’s food intake, body weight and body weight coefficient increased, the feed conversion rate increased, and abdominal fat accumulated. Estrogenic group, soybean extract group and daidzein group all reduced the energy metabolism caused by ovariectomy in different degrees, but soybean polysaccharide had no effect. Conclusion: Ovariectomized rats can be used as an animal model for menopausal women’s obesity. Soybean extract can reduce the pathological changes such as increased body weight, increased food intake and feed conversion caused by ovariectomy. The active ingredients are flavonoids.