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1984年4月～1987年4月我们用鼠疫EV菌感染的小白鼠饲喂草原雕(Aquila rapax nipa-lensis)进行了鼠疫模拟感染试验。结果,在收集的吐物中进行鼠疫FI抗原及鼠疫菌检测均为阴性。从受试的草原雕血清中也未能检出FI抗体。用鼠疫EV菌经草原雕皮下注射感染,在其血清中能检出FI抗体,且至少可保持240天。用不同N浓度的HCI及NaOH溶液水解鼠疫EV菌,对用鼠疫EV菌感染的小白鼠在2N以上的HCl及0.4N以上的NaOH溶液中作用12小时,可使小白鼠全部或部分水解,用抗体中和、反向血凝以及细菌学方法对上述溶液进行检测,在1N以上的HCl与0.4N以上的NaOH水解液中未能检测到FI抗原,细菌培养阴性。而在1N以下的HCl与0.4N NaOH以下的水解液中,则能检测到FI抗原,并培养出鼠疫菌。在鼠疫EV菌菌体的水解液中,检出FI抗原及鼠疫菌的N浓度比前者稍低。实验结果证明足够N浓度的酸、碱溶液,作用于EV菌体或感染鼠,均能使菌体蛋白质分解,而丧失抗原性。
From April 1984 to April 1987 we tested the plague plague infection in mice infected with plague EV-infected mice (Aquila rapax nipa-lensis). As a result, plague FI antigen and Yersinia pestis test were negative in the collected spit. FI antibodies were also not detected from the tested grass carp serum. Infection with plague EV bacteria by subcutaneous injection of grass carcasses, FI antibodies can be detected in their serum, and can be maintained for at least 240 days. Using different concentrations of HCI and NaOH solution hydrolyzed plague EV bacteria, mice infected with the plague EV bacteria in more than 2N HCl and 0.4N above NaOH solution for 12 hours, the mice can be fully or partially hydrolyzed with Antibody neutralization, reverse hemagglutination and bacteriological methods to detect the above solution, in the more than 1N HCl and 0.4N NaOH hydrolyzate failed to detect the FI antigen, bacterial culture negative. In the 1N HCl and 0.4N NaOH below the following hydrolyzate, you can detect the FI antigen, and the cultivation of Yersinia pestis. In the hydrolyzate of the plague EV bacterium, the concentrations of the N-antigen of FI and Y. pestis were detected slightly lower than the former. Experimental results show that enough N concentration of acid and alkali solution, acting on the EV bacteria or infected mice, can make bacterial protein decomposition, and loss of antigenicity.