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植物基因工程包括受体系统的建立,目的基因的分离,载体的构建,转化方法的研究和基因的导入、整合和表达等诸多方面互相联系的研究。中国科学院遗传所田文忠、李良材、陈 英实验室在“六五”期间即得到原国家科委的资助,开始了建立水稻、小麦的转化受体系统的研究。1986年开始承担国家“七五”攻关课题“水稻转化系统的建立”,1987年又承担了国家“863”高科技项目“禾谷类转化系统研究”的课题,并于同年参加了美国洛氏基金会组织的水稻生物技术国际合作项目。主要的研究对象是水稻,也扩展到小麦和玉米。 此论文集收集了作者在这一领域中十几年来发表的主要文章,从中可以看出其研究工作的发展过程,总的说来当时是站在国际研究的前沿,对建立一个高效的禾谷类作物的转化系统,特别是水稻的转化系统,作出了应有的贡献。目前,获得的抗稻瘟病等转基因株系已经进入产业化阶段。
Plant genetic engineering includes the establishment of the receptor system, the separation of the target gene, vector construction, transformation methods and gene introduction, integration and expression and many other related research. Tian Wenzhong, Li Liangcai and Chen Ying of the Institute of Genetics, Chinese Academy of Sciences received the funding from the former State Science and Technology Commission during the “Sixth Five-year Plan” and started the research on establishing the transformation receptor system of rice and wheat. In 1986, he undertook the national “Seventh Five-Year” research project “Establishment of Rice Transformation System”, and undertook the subject of “National 863 High-tech Research Project on Cereal Transformation System” in 1987. In the same year, he participated in the United States Rockwell Fund Will be organized by the international cooperation in rice biotechnology projects. The main research object is rice, but also extends to wheat and corn. This essay collects the major articles published by the author in this field for more than a decade, from which it can be seen that the development of its research work was generally at the forefront of international research, and it was of great significance to establish an efficient cereal Crop transformation system, especially rice transformation system, made its due contribution. At present, the obtained transgenic lines such as blast resistance have entered the stage of industrialization.