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目的:体外试验探讨BMP-4及Smad1介导的信号通路在促进头颈鳞癌侵袭中的作用。方法:采用人重组BMP-4干预头颈鳞癌细胞(SCCHN)株,激光共聚集显微镜观察SCCHN细胞发生上皮-间质转化(EMT)的形态学改变。采用免疫印迹法检测蛋白的表达;侵袭试验用以检测细胞的侵袭力与迁移力的改变。结果:BMP-4可促进SCCHN细胞发生EMT形态学改变,同时促进细胞发生侵袭,EMT相关蛋白E-caherin显著下调,vimentin显著上调;Smad1磷酸化水平增高。结论:BMP-4可通过诱导头颈鳞癌细胞发生EMT从而促进肿瘤的转移,为头颈鳞癌转移机制研究开辟新的通路。
OBJECTIVE: To investigate the role of BMP-4 and Smad1-mediated signal transduction in promoting the invasion of head and neck squamous cell carcinoma in vitro. Methods: Human head squamous cell carcinoma (SCCHN) cells were treated with recombinant BMP-4. The morphology of epithelial-mesenchymal transition (EMT) in SCCHN cells was observed by laser confocal microscope. Immunoblotting was used to detect the protein expression; invasion assay was used to detect cell invasion and migration changes. RESULTS: BMP-4 promoted the morphological changes of EMT in SCCHN cells and promoted the invasion of cells. EMT-associated protein E-caherin was significantly down-regulated and vimentin was up-regulated. The phosphorylation of Smad1 was increased. Conclusion: BMP-4 can induce tumor metastasis by inducing EMT in head and neck squamous cell carcinoma, which opens a new pathway for the study of metastasis mechanism of head and neck squamous cell carcinoma.