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Objective To explore gene expression of NANOG in T-cell acute lymphoblastic leukemia(T-ALL)cell lines and the effects of NANOG gene down-regulation on apoptosis of leukemia cells.Methods Real-time PCR(RtPCR)and Western blot were used to detect the expression level of NANOG gene and protein in MOLT-4,CCRF-HSB2 and Jurkat cells.
Objective To explore gene expression of NANOG in T-cell acute lymphoblastic leukemia (T-ALL) cell lines and the effects of NANOG gene down-regulation on apoptosis of leukemia cells. Methods Real-time PCR (RtPCR) and Western blot were used to detect the expression level of NANOG gene and protein in MOLT-4, CCRF-HSB2 and Jurkat cells.